Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress

doi: 10.1155/2012/681328

Figure Lengend Snippet: 2-DE gel analysis of protein expression in testes of rats treated with H 2 O 2 and H 2 O 2 plus TSL-A. Protein expressions in testes of SD rats IP injected with 1 mmol H 2 O 2 /kg b.w every other day (a) and fed with normal diet, or TSL-A (13 mg/kg b.w/day) (b) for 8 weeks, were separated by 2-DE. Protein spots with numbers represented the seven-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in .

Article Snippet: The 2-DE reagent including acrylamide solution (25%) thiourea, immobiline dry strips, immobilized pH gradients (IPG) buffer (pH = 3–10), IPG cover mineral oil, iodoacetamide (IAA), TEMED, trifluoroacetic acid (TFA), 2-DE clean-up kit, 2-DE Quant kit, and silver staining kit were purchased from GE healthcare (Piscataway, NJ, USA).

Techniques: Expressing, Injection, Software

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Toona sinensis Leaf Aqueous Extract Improves the Functions of Sperm and Testes via Regulating Testicular Proteins in Rats under Oxidative Stress

doi: 10.1155/2012/681328

Figure Lengend Snippet: Alterations of 7 identified protein expressions from 2-DE gel. Protein expressions in testes of SD rats IP injected with 1 mmol H 2 O 2 /kg b.w every other day and fed with normal diet and TSL-A (13 mg/kg b.w/day) for 8 weeks were separated by 2-DE. Protein spots with numbers represented the 7-protein expression more than 2-fold analyzed by the ImageMaster 2D Platinum Software. Details of the proteins were given in . The symbols “+” and “−” represent the upregulation and downregulation of the protein expression, respectively. The expression fold was that of the H 2 O 2 group.

Article Snippet: The 2-DE reagent including acrylamide solution (25%) thiourea, immobiline dry strips, immobilized pH gradients (IPG) buffer (pH = 3–10), IPG cover mineral oil, iodoacetamide (IAA), TEMED, trifluoroacetic acid (TFA), 2-DE clean-up kit, 2-DE Quant kit, and silver staining kit were purchased from GE healthcare (Piscataway, NJ, USA).

Techniques: Injection, Expressing, Software